International Journal of Science and Technology

International Journal of Science and Technology>> Volume 7, Number 7, July 2017

International Journal of Science and Technology


Synthesis of Sugar Ester by Local Yeast Lipase in Solvent Free System

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Author Tanathorn Vitisant, Warawut Chulalaksananukul, Rungtiwa Piumthongkum,Nusara Sinbuathong, Puttachad Mekthong, Suphang Chulalaksananuku
On Pages 773-777
Volume No. 2
Issue No. 11
Issue Date November, 2012
Publishing Date November, 2012
Keywords Lipase, Yeast, Sugar ester, Solvent free system


Abstract

Lipase was produced from yeast isolation located in petroleum contaminated soil. This lipase has good stability at pH between5.0-7.0 and at temperature up to 70 o C for 30 minutes. It could tolerate in many organic solvents as acetonitrile or hexane. The highest activity throughput the hydrolysis, as well as, the esterification reaction was expressed at pH 6.0 and temperature at 37 o C. For instant, the application to synthesis of sugar ester from sugars and fatty acids were carried out in various solvent by incubation at 37 o C for 24 hours with agitation at 600 rpm. The effect of types of substrates as sugar (glucose, fructose, sucrose, xylose and sorbitol), and fatty acid (oleic acid, palmitic acid, myristic acid, lauric acid, and palm fatty acid distillate) were conducted. The molar ratio of substrates, as well as the percentage of enzyme involved in the reaction, disclosed to the percentage of yield of sugar ester was recorded. After incubation, the product was separated and analysis with HPLC. The results revealed the successfully catalysis the reaction to sugar ester was achieved in aqueous solution. The suitable initial substrates were glucose and oleic acid. The optimum molar ratio of sugar to fatty acid was at 3 to 1 with the maximum percentage of yield at 94.31 % within 24 hours.

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