International Journal of Science and Technology

International Journal of Science and Technology>> Volume 7, Number 9, September 2017

International Journal of Science and Technology

In vitro Antibacterial activity and Phytochemical screening of the Zimbabwean endemic Aloe ortholopha Christian & Milne-Readhead (Aloaceae).

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Author Zimudzi, C., Mandebvu, R.S., Kunonga, N. Jere, J.,Kativu, S.
On Pages 778-782
Volume No. 2
Issue No. 11
Issue Date November, 2012
Publishing Date November, 2012
Keywords Aloe ortholopha, endemic, phytochemicals, antibacterial activity, clinical bacteria.


Methanol leaf extracts of a Zimbabwean endemic plant Aloe ortholopha were investigated for their phytochemical profile and antibacterial properties. Qualitative phytochemical analyses revealed the presence of tannins, terpenoids, reducing sugars, saponins and flavonoids and absence of alkaloids, anthraquinones and cardiac gylcosides. Antibacterial activity of the extract was evaluated on four clinical bacteria using the agar disk diffusion method. Extracts showed activity against Pseudomonas aeruginosa, Salmonella enterica and Staphylococcus aureus and no activity against Corynebacterium diphtheriae. P. aeruginosa showed the highest susceptibility to the extract with a diameter zone of inhibition of 13.5mm, then S. enterica (11mm) and S. aureus (9.5mm). C. diphtheriae was resistant to the extract. Minimum inhibitory concentration (MIC) values were high ranging from 133 mg/ml in P. aeruginosa, S. enterica and S. aureus to 267 mg/ml in C. diphtheriae. The results of this study reveal the presence of several phytochemicals with proven antibacterial properties in A. ortholopha, thus validating the traditional uses of the plant in treating bacterial infections.

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