International Journal of Science and Technology

International Journal of Science and Technology>> Volume 7, Number 7, July 2017

International Journal of Science and Technology


Phenotypic Detection of Carbapenemases in Members of Enterobacteriacea in Kano, Nigeria

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Author Yusuf, I., Magashi, A.M., Firdausi, F.S. Sharif, A.A., Getso, M. I., Bala J.A, Aliyu, I. A.
On Pages 802-806
Volume No. 2
Issue No. 11
Issue Date November, 2012
Publishing Date November, 2012
Keywords Detection, Prevalence, Carbapenemases, Enterobacteriacea, Meropenem, Imipenem, Kano.


Abstract

Background and Objectives: A study was carried out to determine the occurrence and prevalence of carbapenemase production among members of Enterobacteriacea in Aminu Kano Teaching Hospital (AKTH) Kano-Nigeria. Materials and Method: A total of 135 isolates of Escherichia coli, Klebsiella pneumoniae, Proteus spp., and Serratia spp were screened for susceptibility to Meropenem and Imipenem (10µg, Oxoid, England) by disk diffusion assay in order to determine suspected carbapenemase producing isolates. Isolates with zones of inhibition to Meropenem measuring < 21mm were subjected to confirmatory test using Modified Hodge’s Test. Results: Results show that only 8.9% (16/135) of the isolates were susceptible to Meropenem while 92.6% (125/135) were susceptible to Imipenem. The prevalence of carbapenemases among Enterobacteriaceae was 14.0% (19/135) and the highest prevalence was found among K. pneumoniae (16.7%; 2/12), followed by Proteus spp. (16.0%; 4/25), and E. coli (12.5%; 13/96). No carbapenemases were detected in Serratia spp. All the carbapenemase producing bacteria were resistant to Meropenem and sensitive to Imipenem. Conclusion: This study therefore showed that carbapenemases occurs in some members of Enterobacteriacea in Kano, Nigeria. The enzymes occur at an alarming rate in Kano, raising fear of multiple drug resistance by Gram negative bacterial pathogens.

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