International Journal of Science and Technology

International Journal of Science and Technology>> Volume 7, Number 9, September 2017

International Journal of Science and Technology


One – Factor – At –A- Time Optimization of Spectrophotometric Method for the Determination of Diclofenac in Pharmaceutical Preparations

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Author Tukur Bala, M. S. Sallau, A. A. Nuhu
Volume No. 5
Issue No. 9
Issue Date September 01, 2015
Publishing Date September 01, 2015
Keywords Method development, pharmaceutical formulation, analysis, optimization, diclofenac


Abstract

One – factor – at – a – time optimization procedure (OFAT) was developed for spectrophotometric determination of diclofenac sodium in pharmaceutical preparations using potassium permanganate to form complex compound in the presence of slightly acidic medium and the complex form of the drug was monitored at a wavelength of 455nm. The method was validated and successfully applied to the assay of 11 brands of the pharmaceutically formulated drugs. Calibration curve was linear within the range of 5µg/L - 35µg/L with correlation coefficient (r2) of 0.994 respectively. The procedure was precise, as determined by %RSDof 0.3% and 0.1% for intra-day (repeatability) and inter day precision (reproducibility) respectively. Limit of detection and limit of quantification was found to be 0.13mg/L and 0.43mg/L respectively. Recoveries were obtained as 85.86 – 110.53%. The statistical paired t – test showed that there was no significant difference comparing the developed method with the official (BP) method. Chauvenet’s criterion result revealed that “sortenforte” was an outlier and therefore a possible counterfeit or substandard formulation. The newly developed method compared favorably and can effectively serve as alternative to the contemporary method for routine determination of the analyte in pharmaceutical formulations.

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